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商品編號


GK80110



品牌


ProZyme



公司


ProZyme, Inc



公司分類(lèi)


Glyko Tools



Size

1 Kit

商品信息

Description

(formerly GE50)

Contains all enzymes & reagents needed to remove all N-linked glycans & simple O-linked glycans (including polysialylated) from glycoproteins. See the
product manual
for an example using bovine fetuin.

N-Glycanase (PNGase F) cleaves all N-linked complex, hybrid or high mannose oligosaccharides unless α(1,3) core fucosylated (asparagine must be peptide-bonded at both termini).

Unfortunately there is no such “universal” endoglycosidase for O-glycans, these have to be trimmed down to Gal-GalNAc-O-S/T before O-glycanase (
GK80090
) can remove the Gal-GalNAc structure. Modification of this core with e.g. sialic acid will block O-glycanase.
Sialidase A (
GK80040
) is included in the kit and can?remove terminal sialic acid from sialylated T antigen/Core 1 (Sia-Gal-GalNAc-O-S/T) to reveal core 1 Gal-GalNAc. The?
GK80115
extender kit (β(1,4)Galactosidase & β-N-Acetylglucosaminidase), available separately, can assist?with removal of e.g. core 2 structures (GlcNAc-Gal-GalNAc-S/T). However, for more extended & complex O-glycans structures these enzymes will not be sufficient to reveal Gal-GalNAc. For more on O-GalNAc glycan core structures, please see reference [1].

Size:
1 kit (~2 mg of glycoprotein)

Product Code:
GK80110

1.?
Brockhausen I
,
Schachter H
, and
Stanley P
.?Essentials of Glyco
BIOLOG
y. 2nd edition.?Cold Spring Harbor (NY):
Cold Spring Harbor Laboratory Press
; 2009.
Chapter 29,?O-GalNAc Glycans
.