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商品編號


Q33226



品牌


ThermoFisher(賽默飛世爾)



公司


Thermo Fisher Scientific



公司分類(lèi)


生命科學(xué)


商品信息

描述
The
qubit
4 Flurometer is the latest version of the popular
qubit
fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive
qubit
assays. The
qubit
4 Fluorometer was re-engineered to run the
qubit
RNA IQ (integrity & quality) assay. The
qubit
4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps.
For all
qubit
assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.
Key features of the
qubit
4 Fluorometer include:
? Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
? Measures intact RNA in less than 5 seconds per sample
? High levels of accuracy using only 1–20 μL of sample, even with very dilute samples
? On-board Reagent Calculator quickly generates
qubit
working solution preparation instructions
? Stores results from up to 1000 samples
? Large 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
? Graphical display indicates when samples are in the extended range or out of range
? Small footprint saves space on your bench
? Exports data to a USB drive or directly to your computer via a USB cable
?
ABI
lity to personalize your
qubit
fluorometer with the assays you run most, add new assays, or even create your own assays with the My
qubit
software and web tool
? Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese
The
qubit
4 Flurometer can also be used to directly measure the fluorescence of samples using Fluorometer mode. Additionally, Ion Sphere Particle quality can be evaluated on the
qubit
4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM sequencer.
Enables more accurate results than with UV absorbance at low concentrations
The
qubit
4 Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the
qubit
fluorometer, your research is enhanced by more accurate measurements because the dyes in the
qubit
assay kits fluoresce only when bound to the selected molecule (DNA, RNA or protein) in your sample. This allows you to avoid repeating work due to inaccurate measurements.
Fast, simple, and accurate measurement of RNA quality
The
qubit
RNA IQ assay provides a fast, simple method to check whether an RNA sample has degraded. The assay utilizes two unique dyes: one that binds to large, intact RNA, and another that selectively binds to small, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample. To use, simply add your samples to the RNA IQ working solution, then measure on the
qubit
4 Fluorometer.
Save samples and bench space
The thoughtfully designed
qubit
4 Fluorometer requires samples of only 1–20 ?L. It is intended for use at room temperature and occupies only a small amount of bench space. The
qubit
4 Fluorometer features advanced optics and data analysis algorithms, and comes equipped with a USB thumb drive and cable for data transfer to Excel? software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification.
Single sample measurements are a breeze
This user-friendly fluorometer is combined with quick and simple assay procedures. Calculations and settings are automatically performed by the instrument. The
qubit
assays for use with the
qubit
4 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays. 500 ?L thin-walled PCR tubes (Cat. No.
Q32856
) are required.
System specifications include:
Instrument dimensions:
?
5.4"(w) x 10" (l) x 2.2" (h) (13.6 cm x 25 cm x 5.5 cm)
Weight:
?
743 g
Dynamic range:
?
5 orders of magnitude
Processing time:
?
≤seconds/sample
Light sources:
?
blue LED (max ~470 nm), red LED (max ~635 nm)
Excitation filters:
?
blue (430–495 nm), red (600–645 nm)
Emission filters:
?
green (510–580 nm), red (665–720 nm)
Detectors:
?
photodiodes, measurement cap
ABI
lity from 300–1,000 nm
Warm-up time:
?
<35 seconds
USB thumb drive:
?
4 GB