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商品編號


Leica TCS SP8 X



品牌


Leica(徠卡)



公司


Leica



公司分類(lèi)


Confocal Microscopes





商品信息

The white light laser source of the
Leica
TCS SP8 X
perfectly matches
the
wavelength
of any

fluorophore. Up to
eight excitation lines
can be used – simultaneously.

By tuning both excitation
and
detection, complete
excitation emission spectra
can be acquired.

With this spectral information, any dye can be optimally excited with
minimum cross-excitation
and specimen damage.

In addition, the pulsed
White Light Laser (WLL)
supplies excitation light for
fluorescence lifetime measurements
.

FLIM
provides information about the direct environment of a molecule, like local pH or binding to another molecule.










Complete spectral freedom



The choice of fluorescent labels is not limited any longer by the hardware. The researcher selects the dyes according to their avail
ABI
lity or the scientific needs. With up to five spectral detection channels, the
Leica
TCS SP8 X is completely tunable and fully adapts to the specimen.












Full spectral characterization of images



New dyes or fluorescent proteins can be discovered and characterized. A two-dimensional excitation-emission spectrum characterizes the spectral properties of any fluorescent sample. Fluorophores with identical emission or excitation spectra are discriminated. This is essential for multi-color fluorescence experiments.














Zero background by LightGate



LightGate is a clever, filter-free method to remove unwanted signal from the image data. An adjustable time gate switches off the data collection during the white light laser pulse. Additionally, non-wanted fluorescence can be removed by adjusting the time gate. This way the highest image contrast without any reflection contribution is obtained.












Free selection of FLIM excitation wavelength



The White Light Laser is an appropriate excitation source for fluorescence lifetime measurements. Its
continuous tun
ABI
lity
allows performing FLIM measurement at
any excitation wavelength
. Lifetime of
orange and red dyes
can now be obtained. The optimal laser repetition rate is selected via an integrated pulse picker.