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商品編號


GSC3002G



品牌


Stemgent



公司


Stemgent, Inc



公司分類(lèi)


Cell Lines



Size

4-5 x 100
6
cells/vial

商品信息


Product Overview

Neonatal human foreskin fibroblasts (NuFFs) are mitotically inactivated fibroblasts produced by GlobalStem and functionally validated by
Stemgent
to support the generation of induced pluripotent stem (iPS) cell colonies from human newborn foreskin fibroblasts using the
Stemgent
mRNA Reprogramming Kit. NuFF-RQ cells can also be used to condition?Pluriton? Reprogramming Medium?and improve the productivity of iPS generation in feeder-free and extended feeder-based reprogramming cultures.






Product Specifications


Size

4-5 x 10
6
cells per vial

Species


Homo sapien

Source

Normal, Neonatal Foreskin Fibroblasts, donor 12

Storage and St
ABI
lity

Store in vapor phase liquid nitrogen. Stable for a minimum of 6 months from date of receipt when stored as directed.

Quality Control?

Cells tested negative for mycoplasma, bacteria, fungi, and spore contamination. When thawed and cultured according to
Stemgent
protocols NuFF-RQ cells are guaranteed to be greater than 80% viable upon thaw.

Propagation

For use as a feeder layer and/or conditioning Pluriton? Reprogramming Medium, please reference
User Manual:
Stemgent
mRNA Reprogramming System
and/or
Protocol:?Generating NuFF-Conditioned Pluriton Medium
.

Notice to Purchaser

Purchaser represents and warrants that it will use NuFF-RQ IRR?cells purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications.
Stemgent
will not be held respons
IBL
e for patent infringement or other violations that may occur with the use of our products.

Restricted Use

For restrictions related to the use of this product please refer to the accompanying Restricted Use Licence.





Specification Sheets
PS-CL-GSC3002G
Safety Data Sheets
GSC-3002G Safety Data Sheet
Product Sheets
Product Sheet_RNA Reprogramming



Related Products

microRNA Booster Kit (00-0073)
mRNA Reprogramming Kit (00-0071)
Reprogramming Medium (00-0070)



References


Warren, L., Manos, P.D., Ahfeldt, T., Loh, Y.H., Li, H., Lau, F., Ebina, W., Mandal, P.K., Smith, Z.D., Meissner, A., Daley, D.Q., Brack, A.S., Collins, J.J., Cowan, C., Schlaeger, T.M., and Rossi, D.J. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7: 618-630.