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商品編號


3860-2A



品牌


Mabtech



公司


Mabtech



公司分類(lèi)


Human



Size

for 4 plates














商品信息
Basic ELISpot for enumeration of IgA secreting B cells
Human IgA ELISpot
BASIC
enables enumeration of B cells secreting total human IgA as well as antigen-specific human IgA. It contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-ALP and polyclonal activators.
Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
Human IgA ELISpot
BASIC
is also available with Streptavidin-HRP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.

Analyte Information

IgA
IgA (Immunoglobulin A) represents only 10-15% of the total Ig molecules in serum but is the predominating Ig isotype in saliva, tears, breast milk and in the gastrointestinal and urogenital tract. In serum, the IgA molecule is commonly found as a monomer of either IgA1 or IgA2 subclass but can also exist as dimers or larger polymeric forms. The IgA found in secretions (secretory IgA or sIgA) is commonly a dimer with two IgA molecules joined by the J chain. Secretory IgA is the most important Ig isotype in mucous surfaces, the area of entry for most pathogens.
Application:


ELISpot


Reactivity:


Human


Contents:


Capture mAb (MT57)
Biotinylated detection mAb (MT20)
Biotinylated detection mAb (MT57)
Streptavidin-ALP
R848
Recombinant human IL-2


Size:

for 4 plates

Other formats:

Click to view all Human IgAproducts






I have compared
Mabtech
ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples.
Mabtech
's kits proved to be superior
to our protocol, had significantly lower background and provided better and higher detection of spots.








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Mabtech/Human IgA ELISpot
BASIC
(ALP)/3860-2A/for 4 plates






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