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商品編號


3850-2A



品牌


Mabtech



公司


Mabtech



公司分類(lèi)


Human/NHP



Size

for 4 plates














商品信息
Basic ELISpot for enumeration of IgG secreting B cells
Human IgG ELISpot
BASIC
enables enumeration of B cells secreting total IgG as well as antigen-specific IgG. It contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-ALP and polyclonal activators.
Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
IgG ELISpot
BASIC
is also available with Streptavidin-HRP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.
Species cross-reactivity
The monoclonal antibodies cross-react with IgG from non-human primates. R848 and human IL-2 can be used for stimulation of B cells from non-human primates See the Human/NHP cross-reactivity table.

Analyte Information

IgG
IgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. In mice the IgG subclasses are defined IgG1, IgG2a/c, IgG2b and IgG3. The IgG molecule consists of two heavy and two lights chains (κ or λ) resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.
Application:


ELISpot


Reactivity:


Human / NHP




Contents:


Capture mAbs (MT91/145)
Biotinylated detection mAbs (MT78/145)
Streptavidin-ALP
R848
Recombinant human IL-2


Size:

for 4 plates

Other formats:

Click to view all Human/NHP IgGproducts






I have compared
Mabtech
ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples.
Mabtech
's kits proved to be superior
to our protocol, had significantly lower background and provided better and higher detection of spots.








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Mabtech/Human IgG ELISpot
BASIC
(ALP)/3850-2A/for 4 plates






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