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商品編號


3880-2H



品牌


Mabtech



公司


Mabtech



公司分類(lèi)


Human/NHP



Size

for 4 plates














商品信息
Basic ELISpot for enumeration of cells secreting IgM
Human IgM ELISpot
BASIC
enables enumeration of B cells secreting total human IgM as well as antigen-specific human IgM. It contains matched quantities of monoclonal capture and detection antibodies, Streptavidin-HRP and polyclonal activators.
Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
Human IgM ELISpot
BASIC
is also available with Streptavidin-ALP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.

Analyte Information

IgM
Monomeric IgM (Immunoglobulin M) is found on the surface of B cells whereas the IgM molecules secreted from plasma B cells are pentamers, i.e. five IgM molecules joined together by the J chain. IgM constitutes approximately 10% of the total Ig concentration in serum. IgM is the first antibody isotype induced in a typical immune response and hence is the isotype with the lowest affinity for antigens. Due to its structure, with 10 antigen-binding sites per IgM pentamer, it can nevertheless bind e.g. surface-bound antigens with a very high
Avidity
.
Application:


ELISpot


Reactivity:


Human / NHP




Contents:


Capture mAbs (MT11/12)
Biotinylated detection mAb (MT22)
Streptavidin-HRP
R848
Recombinant human IL-2


Size:

for 4 plates

Other formats:

Click to view all Human/NHP IgMproducts






I have compared
Mabtech
ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples.
Mabtech
's kits proved to be superior
to our protocol, had significantly lower background and provided better and higher detection of spots.








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Mabtech/Human IgM ELISpot
BASIC
(HRP)/3880-2H/for 4 plates






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