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商品編號


3450-4APW-10



品牌


Mabtech



公司


Mabtech



公司分類(lèi)


Human/NHP



Size

10 plates














商品信息
ELISpot
PLUS
for enumeration of cells secreting IL-12/-23 (p40)
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human IL-12/-23 (p40). The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate.
The monoclonal antibodies in this kit are specific to the p40 subunit of IL-12 and IL-23. ELISpot assays based on antibodies to p40 will detect both IL-12 and IL-23. Such assays can also detect p40 monomers and dimers.
Pre-coated plates reduce assay time and minimize vari
ABI
lity.
Human IL-12/-23 (p40) ELISpot
PLUS
kits are also available with Streptavidin-HRP and TMB substrate. See Related products.
Species cross-reactivity
The monoclonal antibodies in this kit cross-react with IL-12/-23 (p40) from non-human primates. See the Human/NHP cross-reactivity table.

Analyte Information

IL-12/-23 (p40)
Interleukin-12 (IL-12, IL12) is, when
BIOLOG
ically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing proliferation and cytotoxicity of NK and T cells. Interleukin-23 (IL-23) is a disulfide-linked heterodimer which consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells and IL-23 is respons
IBL
e for Th17 expansion and st
ABI
lization. Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.
Application:


ELISpot


Reactivity:


Human / NHP




Contents:


Biotinylated detection mAb (MT618)
Streptavidin-ALP
Substrate (BCIP/NBT-plus)
10
Pre-coated white plates (mAbs MT86/221)


Size:

10 plates

Other formats:

Click to view all Human/NHP IL-12/-23 (p40)products






I have compared
Mabtech
ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples.
Mabtech
's kits proved to be superior
to our protocol, had significantly lower background and provided better and higher detection of spots.








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Mabtech/Human IL-12/-23 (p40) ELISpot
PLUS
(ALP)/3450-4APW-10/10 plates






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