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商品編號


AG-40B-0010-C010



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Immunology



Size

10 ?g


商品信息


More Information



Product Details



Synonyms

MultimericCD40L?; ACRP30
headless
:CD40L; ACRP30
headless
:CD154; ACRP30
headless
:TNFSF5;
ADI
POQ-CD40L



Product Type

Protein



Properties



Source/Host

CHO cells



Sequence

Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30
headless
(aa 18-111) and a FLAG
?
-tag.



Crossreactivity

Human



Specificity

Binds to human CD40.



BIOLOG
ical Activity

Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).



MW

~35-40kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.01eu>



Concentration

0.1mg/ml after reconstitution.



Reconstitution

Reconstitute with 100μl sterile water.



Formulation

Lyophilized. Contains PBS



Other Product Data


UniProt link P29965: CD40L (human)
UniProt link Q60994: ACRP30 (mouse) (precursor)

FLAG is a registered trademark of
Sigma-Aldrich
Co.




Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





MultimericCD40L? is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L
in vivo
. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L? has shown to suppress alum-induced IL-1β release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM vi
ABI
lity. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.


Product References

IgG subclass switch capacity is low in switched and in IgM-only, but high in IgD+IgM+, post-germinal center (CD27+) human B cells: C. Werner-Favre, et al.; Eur. J. Immunol.
31,
243 (2001)

Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol.
23,
1428 (2003)

Impaired CD40L signaling is a cause of defective IL-12 and TNF-alpha production in Sezary syndrome: circumvention by hexameric soluble CD40L: L.E. French, et al.; Blood
105,
219 (2005)

Cysteine-rich Domain 1 of CD40 Mediates Receptor Self-assembly: C.R. Smulski, et al.; J. Biol. Chem.
288,
10914 (2013)

Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay: H.B. Larman, et al.; Nucl. Acids Res.
42,
9146 (2014)

CD4+ T Cell-derived IL-21 and deprivation of CD40 signaling favor the In Vivo development of granzyme B-expressing regulatory B cells in HIV patients: C. Kaltenmeier, et al.; J. Immunol.
194,
3768 (2015)