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商品編號


YIF-LF-R0001



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Cell Lines



Size

200 ?l

商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

HeLa cells



Crossreactivity

Human



Application

Western Blot



Concentration

1mg/ml



Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Boil for 5minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Immunoblot analysis.



Declaration

Manufactured by AbFrontier



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate. Cells were lysed in Lysis buffer (20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstatin A, 5ug/ml Aprotinin, 5ug/ml Leupeptin) and diluted with reducing sample buffer (60mM Tris, pH 6.8, 6% glycerol, 2% SDS, 0.2% bromphenol blue, 200mM 2-mercaptoethanol).