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商品編號


CHI-AG-40A-0079-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Immunology



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Fractalkine; Chemokine (C-X3-C Motif) Ligand 1; Neurotactin; SCYD1



Product Type

Protein



Properties



Source/Host

HEK 293 cells



Sequence

The extracellular domain of human CX3CL1 (aa 25-339) is fused at the C-terminus to a FLAG
?
-tag.



Crossreactivity

Human



MW

~50kDa (SDS-PAGE)



Purity

≥90% (SDS-PAGE)



Endotoxin Content

<0.06eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.06eu>



Reconstitution

Reconstitute 10?g vial with 100 ?l sterile water to a concentration of 0.1mg/ml.
Reconstitute 50?g vial with 100 ?l sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.



Accession Number

AAH01163.1



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Other Product Data


UniProt link AAH01163.1: CX3CL1 (Fractalkine) (human)
FLAG is a registered trademark of
Sigma-Aldrich
Co.




Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





CX3CL1 [Fractalkine] is expressed on TNF-α and IL-1 activated endothelial cells (ECs) and binds to CX3CR1. CX3CL1 can exist in 2 forms, as membrane-anchored or as a shed 95-kDa glycoprotein. The soluble CX3CL1 has potent chemoattractant activity for T cells and monocytes, and the cell surface bound protein, which is induced on activated primary endothelial cells, promotes strong adhesion of those leukocytes. CX3CL1 may play a role in regulating leukocyte adhesion and migration processes at the endothelium.