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商品編號


INTCYSPAI-L



品牌


Innovative Research



公司


Innovative Research



公司分類(lèi)


Proteins



Size

1.0mg

商品信息

Product Description
Human PAI-1 N-terminal cysteine mutant, latent fraction
Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The latent fraction is < 0.4 percent active as determined by titration with HMW tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
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