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商品編號


3443-003-01



品牌


Trevigen



公司


Trevigen, Inc



公司分類(lèi)


Extracellular Matrix Proteins



Size

1 ml, 35 ml

商品信息

Description

Cultrex? Rat Collagen I, Lower Viscosity is purified from rat tail tendons?and?is provided at a standard?concentration that is lower in viscosity, making it easier to work with. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 3 mg/ml.

Cultrex? Rat Collagen I, Lower Viscosity is a purified stromal?ECM protein?that has been developed, produced and qualified at a low viscosity for general cell culture applications.

Quality control specifications:


No bacterial or fungal growth detected after incubation at 37°C for 14 days following
USP
sterility testing guidelines.

Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.

No mycoplasma contamination detected by PCR.

Protein concentration of 3 mg/ml.

Provided in?20 mM Acetic Acid, pH 3.4 – 3.6.

Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.

BIOLOG
ical activity determined for each lot:

Cell Attachment: Tested for the
ABI
lity to promote cell attachment and spre
ADI
ng of HT1080 human fibrosarcoma cells.