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商品編號


3442-005-01



品牌


Trevigen



公司


Trevigen, Inc



公司分類(lèi)


Extracellular Matrix Proteins



Size

1 ml, 50 mg

商品信息

Description

Cultrex? Bovine Collagen I?is purified from fetal bovine extensor?tendons?and?is pepsin-treated to remove telopeptides. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Cultrex? Bovine Collagen I?is a purified stromal ECM protein?that has been developed, produced and qualified for general cell culture applications.

Quality control specifications:


No bacterial or fungal growth detected after incubation at 37°C for 14 days following
USP
sterility testing guidelines.

Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.

No mycoplasma contamination detected by PCR.

Protein concentration of 5 mg/ml.

Provided in?20 mM Acetic Acid, pH 3.4 – 3.6.

Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.

BIOLOG
ical activity determined for each lot:

Cell Attachment: Tested for the
ABI
lity to promote cell attachment and spre
ADI
ng of HT1080 human fibrosarcoma cells.






Functionally distinct PI 3-kinase pathways regulate myelination in the peripheral nervous system
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J. Cell Biol., Mar 2014; 204: 1219 – 1236.
http://jcb.rupress.org/cgi/content/abstract/204/7/1219

Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling
Ruei-Zeng Lin, Rafael Moreno-Luna, Dan Li, Shou-Ching Jaminet, Arin K. Greene, and Juan M. Melero-Martin
PNAS, Jun 2014; 10.1073/pnas.1405388111.
http://www.pnas.org/cgi/content/abstract/1405388111v1

Pivotal Role for CD16+ Monocytes in Immune Surveillance of the Central Nervous System
Anne Waschbisch, Sina Schr?der, Dana Schraudner, Laura Sammet, Babette Weksler, Arthur Melms, S
ABI
ne Pfeifenbring, Christine Stadelmann, Stefan Schwab, and Ralf A. Linker
J. Immunol., Feb 2016; 196: 1558 – 1567.
http://www.jimmunol.org/cgi/content/abstract/196/4/1558