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商品編號


AG-20B-0023TS-T100



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Antibodies



Size

100 tests



商品信息


More Information



Product Details



Synonyms

TRAIL Receptor 2; DR5; KILLER; TNFRSF10B; CD262



Product Type

Monoclonal Antibody



Properties



Clone

HS201



Isotype

Mouse IgG1



Source/Host

Purified from concentrated hybridoma tissue culture supernatant.



Immunogen/Antigen

Recombinant human TRAIL-R2 (DR5).



Label/Conjugates

ATTO 647N



Application


Flow Cytometry

Immunocytochemistry

Definition for 1 test:
1μl of labeled antibody in 200μl of Flow Cytometry buffer (see online protocol).



Crossreactivity

Human



Specificity

Recognizes human TRAIL-R2. Does not cross-react with human TRAIL-R1, -R3 or -R4.



Purity

≥95% (SDS-PAGE)



Purity Detail

Protein G-affinity purified.



Concentration

1mg/ml



Formulation

Liquid. In PBS containing 0.02% sodium azide.



Other Product Data

New ATTO-fluorescent antibodies show increased photost
ABI
lity, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization.
ATTO 647N shows red fluorescence (&lam
BD
a;abs (max): 645nm, &lam
BD
a;em (max): 669nm, εmax: 120’000).



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.
Protect from light.



Use/St
ABI
lity

Keep conjugated formats at +4°C.
Stable for at least 1 year after receipt when stored at +4°C.



Documents



Protocols


Download PDF



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





TRAIL-R2 is a receptor for the cytotoxic ligand TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-κB.


Product References

Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8: M.R. Sprick, et al.; EMBO J.
21,
4520 (2002)

Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Signaling in Activated T Cells Abrogates TRAIL-Induced Apoptosis Upstream of the Mitochondrial Amplification Loop and Caspase-8: T.S. Soderstrom, et al.; J. Immunol.
169,
2851 (2002)

Bile acids stimulate cFLIP phosphorylation enhancing TRAIL-mediated apoptosis: H. Higuchi, et al.; J. Biol. Chem.
278,
454 (2003)

Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation: M. Leverkus, et al.; Mol. Cell. Biol.
23,
777 (2003)

TNF-Related Apoptosis-Inducing Ligand Mediates Tumoricidal Activity of Human Monocytes Stimulated by Newcastle Disease Virus: B. Washburn, et al.; J. Immunol.
170,
1814 (2003)

Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs: T.M. Ganten, et al.; Cell Death Differ.
11 Suppl 1,
86 (2004)

Casein kinase I attenuates tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by regulating the recruitment of fas-associated death domain and procaspase-8 to the death-inducing signaling complex: K. Izeradjene, et al.; Cancer Res.
64,
8036 (2004)

Fas-associated protein with death domain (FADD)-independent recruitment of c-FLIPL to death receptor 5: T.G. Jin, et al.; J. Biol. Chem.
279,
55594 (2004)

TRAIL regulates normal erythroid maturation through an ERK-dependent pathway: P. Secchiero, et al.; Blood
103,
517 (2004)

IFN{alpha}-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells: C. Tecchio, et al.; Blood
103,
3837 (2004)

cFLIPL inhibits tumor necrosis factor-related apoptosis-inducing ligand-mediated NF-kappaB activation at the death-inducing signaling complex in human keratinocytes: T. Wachter, et al.; J. Biol. Chem.
279,
52824 (2004)

Resistance to FasL and tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in Sezary syndrome T-cells associated with impaired death receptor and FLICE-inhibitory protein expression: E. Contassot, et al.; Blood
111,
4780 (2008)

TRAIL-R2-specific antibodies and recombinant TRAIL can synergise to kill cancer cells: M.H. Tuthill, et al.; Oncogene
34,
2138 (2015)