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商品編號


P3010-5



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分類(lèi)


Protein Expression / Purification Reagents



Size

5 ml


商品信息

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein with a 4X or 6XHis tag. Nickel XPure Agrose Resin provides the advantages of the Nickel cation with the high flow rates of the highly cross-linked agarose beads. Except for batch purification, this product is specially suitable for large scale His-tagged protein purification using FPLC.

The XPure series of resins are made for large scale and fine purification using a high-performance liquid chromatography (HPLC) system.


Additional Information






Product Name:

Ni-IDA XPure Agarose Resin



Catalog No.:

P3010-5



Size:

5 ml



Bead (Geometry, size):

Spherical, fine: ~ 20-50 ?m



Cross-Linked:

Yes



Ligand:

Iminodiacetic acid (IDA)



Agarose %:

6% Agarose



Binding Capacity:

> 26 mg/ml gel (Binding capacity will vary for each target protein.)



Volume %:

50% (v/v) aqueous s
USP
ension containing 20% Ethanol



Storage Temperature:

2-8 °C



Application:

Batch, Gravity, MPLC and FPLC



PDF Data Sheet:

Datasheet



User's Manual:

User’s Guide



Image(s):




Shipping Method:

Wet ice shipping




Details

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein with a 4X or 6XHis tag. Nickel XPure Agrose Resin provides the advantages of the Nickel cation with the high flow rates of the highly cross-linked agarose beads. Except for batch purification, this product is specially suitable for large scale His-tagged protein purification using FPLC. The XPure series of resins are made for large scale and fine purification using a high-performance liquid chromatography (HPLC) system.

Ube2I was purified with Ni-IDA XPure Agarose Resin using a gravity column
Lane 1: ?Molecular weight
Marker
Lane 2: ?Cell pellet after sonication
Lane 3: ?Supernatant after sonication Lane 4: ?Flow through after binding
Lane 5: Wash Lane 6: ?Elution 1
Lane 7: Elution 2 Lane 8: ?Elution 3
Lane 9: ?Elution 4 Lane 10: ?Elution 5