ubpbio/Ni-IDA XPure Agarose Resin/P3010-25/25 ml
蛋白純化
商品編號
P3010-25
品牌
UBPBio
公司
Ubiquitin-Proteasome Biotechnologies, LLC
公司分類(lèi)
Protein Expression / Purification Reagents
Size
25 ml
商品信息
Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein with a 4X or 6XHis tag. Nickel XPure Agrose Resin provides the advantages of the Nickel cation with the high flow rates of the highly cross-linked agarose beads. Except for batch purification, this product is specially suitable for large scale His-tagged protein purification using FPLC.
The XPure series of resins are made for large scale and fine purification using a high-performance liquid chromatography (HPLC) system.
Additional Information
Product Name:
Ni-IDA XPure Agarose Resin
Catalog No.:
P3010-25
Size:
25 ml
Bead (Geometry, size):
Spherical, fine: ~ 20-50 ?m
Cross-Linked:
Yes
Ligand:
Iminodiacetic acid (IDA)
Agarose %:
6% Agarose
Binding Capacity:
> 26 mg/ml gel (Binding capacity will vary for each target protein.)
Volume %:
50% (v/v) aqueous s
USP
ension containing 20% Ethanol
Storage Temperature:
2-8 °C
Application:
Batch, Gravity, MPLC and FPLC
PDF Data Sheet:
Datasheet
User's Manual:
User’s Guide
Image(s):
Shipping Method:
Wet ice shipping
Details
Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein with a 4X or 6XHis tag. Nickel XPure Agrose Resin provides the advantages of the Nickel cation with the high flow rates of the highly cross-linked agarose beads. Except for batch purification, this product is specially suitable for large scale His-tagged protein purification using FPLC.
The XPure series of resins are made for large scale and fine purification using a high-performance liquid chromatography (HPLC) system.
Ube2I was purified with Ni-IDA XPure Agarose Resin using a gravity column
Lane 1: Molecular weight
Marker
Lane 2: Cell pellet after sonication
Lane 3: Supernatant after sonication
Lane 4: Flow through after binding
Lane 5: Wash
Lane 6: Elution 1
Lane 7: Elution 2
Lane 8: Elution 3
Lane 9: Elution 4
Lane 10: Elution 5
產(chǎn)品貨號:2756.0