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商品編號


P3020-5



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分類(lèi)


Protein Expression / Purification Reagents



Size

5 ml

商品信息

Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90?m beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compat
IBL
e with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.

Additional Information






Product Name:

Ni-NTA XPure Agarose Resin



Catalog No.:

P3020-5



Size:

5 ml



Bead (Geometry, size):

45 - 165 ?m



Cross-Linked:

Yes



Ligand:

Nitilotriacetic acid (NTA)



Agarose %:

Highly cross-linked 6% agarose



Binding Capacity:

> 40mg 6xHis-tagged protein/ml medium



Volume %:

50% (v/v) aqueous s
USP
ension containing 20% Ethanol



Storage Temperature:

2-8 °C



Application:

Batch, Gravity, MPLC and FPLC



PDF Data Sheet:

PDF Datasheet



User's Manual:

PDF User’s Guide



Image(s):

No



Shipping Method:

Wet ice shipping




Details

Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90?m beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compat
IBL
e with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.
The XPure series of resins are made for large scale and fine purification using a high-performace liquid chromatography (HPLC) system.