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商品編號


P3060-100



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分類(lèi)


Protein Expression / Purification Reagents



Size

100 ml


商品信息

Glutathione Agarose Resin consists of glutathione covalently bound to agarose beads. This resin has high binding affinity and specificity to the Glutathione S-transferase (GST) protein. GST-tagged proteins can be highly purified by batch or column purification (low flow rate) in a one step purification method. Bound GST-tagged proteins are easily eluted from the resin with buffers containing reduced glutathione.


Additional Information






Product Name:

Glutathione Xpure Agarose Resin



Catalog No.:

P3060-100



Size:

100 ml



Bead (Geometry, size):

Spherical, Standard: ~ 45-165 ?m



Cross-Linked:

No



Ligand:

Glutathione



Agarose %:

highly cross-linked 4% Agarose



Binding Capacity:

> 10 mg recombinant GST/ml gel (binding capacity may vary for each GST-tagged protein)



Volume %:

50% (v/v) aqueous s
USP
ension containing 20% Ethanol



Storage Temperature:

4-8 °C



Application:

Batch, FPLC



PDF Data Sheet:

Datasheet
,
MSD
S




User's Manual:

User’s Guide



Image(s):




Shipping Method:

Wet ice shipping




Details

Glutathione Agarose Resin consists of glutathione covalently bound to agarose beads. This resin has high binding affinity and specificity to the Glutathione S-transferase (GST) protein. GST-tagged proteins can be highly purified by batch or column purification (low flow rate) in a one step purification method. Bound GST-tagged proteins are easily eluted from the resin with buffers containing reduced glutathione.

GST-E2-25K was purified with Glutathione Agarose Resin using a gravity column.
Lane 1: ?Molecular weight
Marker
Lane 2: ?Cell pellet after sonication
Lane 3: ?Supernatant after sonication Lane 4: ?Flow through after binding
Lane 5: Wash Lane 6: ?Elution 1
Lane 7: Elution 2 Lane 8: ?Elution 3
Lane 9: ?Elution 4 Lane 10: ?Elution 5