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商品編號


AG-40T-0260RH-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

γ-Aminobutyric Acid Type A Receptor Associated Protein; GABA(A) Receptor-associated Protein; Apg8p1; ATG8A; FLC3B; MM46



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human GABARAP (Accession Nr. NP_009209) fused to a N-terminal His-tag and conjugated to Rhodamine.



Crossreactivity

Human



Label/Conjugates

Rhodamine



Formulation

Liquid. In 50mM Hepes pH 7.5, 100mM NaCl.



Other Product Data


Use:
Modified with rhodamine red via primary amine coupling, resulting in the modificationof lysine residues as well as the N-terminus. Allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies. Ideal for use in assays requiring fluorescent detection. Optimal fluorescence at pH 8.0 is monitored with an excitation wavelength of 570nM and an emission wavelength of 590nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-1?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.
Protect from light.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





γ-Aminobutyric Acid Receptor-associated Protein (GABARAP), also known as Apg8p1, is 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the autophagy-related 8 (Atg8) family of proteins. The GABARAP subfamily also includes GABARAPL1. GABARAP has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with ubiquitin, but lack aa sequence similarity. GABARAP was first described for its putative involvement GABA A Receptor trafficking. However, it is best known for its role in autophagy. GABARAP covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a ubiquitin-like conjugation system that includes ubiquitin-activating (E1)-, ubiquitin-conjugating (E2)-, and ubiquitin ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome maturation. It may also be involved in cargo recruitment to autophagosomes.