欧美自拍另类欧美宗合图片区,国产视频一区二区三区四区,日本一区二区三区四区视频,婬片一区AAA毛片一区二区

商品編號


AG-40T-0261-C025



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

γ-Aminobutyric Acid Type A Receptor Associated Protein; GABA(A) Receptor-associated Protein; Apg8p1; ATG8A; FLC3B; MM46



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human GABARAP (Accession Nr. NP_009209) fused to a N-terminal HA-tag and tagged to vinyl sulfone.



Crossreactivity

Human



Formulation

Liquid. In 50mM Hepes pH 7.5, 100mM NaCl, 10% glycerol.



Other Product Data


Use:
Potent, irrevers
IBL
e and specific inhibitor of Agp8-specific isopeptidases (such as Apg4B). Apg4B activities include the processing of Apg8 precursor proteins and the removal of Apg8 proteins that are conjugated to phosphatidylethanolamine during autophagy. These processes can be inhibited by this vinyl sulfone derivative which reacts with the Apg4B active site cysteine. The HA-epitope allows for the sensitive identification or purification of deconjugating activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.Add directly to
in vitro
assay from the stock solution. Depending on conditions and detection method, typical concentrations to fully inhibit Apg deconjugating enzymes
in vitro
are 1-5μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





γ-Aminobutyric Acid Receptor-associated Protein (GABARAP), also known as Apg8p1, is 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the autophagy-related 8 (Atg8) family of proteins. The GABARAP subfamily also includes GABARAPL1. GABARAP has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with ubiquitin, but lack aa sequence similarity. GABARAP was first described for its putative involvement GABA A Receptor trafficking. However, it is best known for its role in autophagy. GABARAP covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a ubiquitin-like conjugation system that includes ubiquitin-activating (E1)-, ubiquitin-conjugating (E2)-, and ubiquitin ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome maturation. It may also be involved in cargo recruitment to autophagosomes.