欧美自拍另类欧美宗合图片区,国产视频一区二区三区四区,日本一区二区三区四区视频,婬片一区AAA毛片一区二区

商品編號


AG-40T-0270-C100



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 B; E2-17k; RAD6B; Ubiquitin-protein Ligase B; UBE2B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human HR6B (Accession Nr. NP_003328) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Homolog of Rad6 B (HR6B), also known as ubiquitin-conjugating Enzyme E2B (UBE2B), is a ubiquitously expressed member of the ubiquitin-conjugating (E2) enzyme family. It has a predicted molecular weight of 17 kDa. The human enzyme shares 100% amino acid sequence identity with the mouse and rat orthologs. HR6B/UBE2B localizes to the nucleus and cytoplasm and is highly expressed in the brain, heart and testis. HR6B/UBE2B has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with ubiquitin. PCNA is mono-ubiquitinated by HR6B/UBE2B via the RAD18 ubiquitin ligase (E3). HR6B/UBE2B also functions with the UBR2 E3 to ubiquitinate Histone H2A. HR6B/UBE2B plays important roles during spermatogenesis and is required for fertility in male mice. Pathologically, HR6B/UBE2B is overexpressed in mouse and human breast cancer cell lines and tumors and has been reported to provide resistance to chemotherapy drugs.