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商品編號


CHI-HF-20107-C010



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

10 ?g

商品信息


More Information



Product Details



Synonyms

Interleukin-7; Lymphopoietin 1; LP-1



Product Type

Protein



Properties



Source/Host

HEK 293 cells



Sequence

The extracellular domain of human IL-7 (aa 26-177) is fused at the C-terminus to a His-tag.



Crossreactivity

Human



BIOLOG
ical Activity

Measured by the dose dependent stimulation of the proliferation of murine 2E8 cells. The ED50 for this effect is typically 0.2ng/ml corresponding to a specific activity of 5x 10
6
units/mg.



MW

~27kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu mu;g="" protein="" (lal="" test;="" lonza).="">0.01eu>



Reconstitution

Reconstitute 10?g vial with 100 ?l sterile water to a concentration of 0.1mg/ml.
Reconstitute 50?g vial with 100 ?l sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Other Product Data

NCBI reference AAH47698.1: IL-7 (human)



Declaration

Manufactured by Chimerigen.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interleukin-7 (IL-7), is a hematopoietic growth factor and a member of the IL-7/IL-9 family. IL-7 stimulates the proliferation of lymphoid
Progen
itors and is secreted by stromal cells in the bone marrow and thymus. IL-7 is also important for proliferation during certain stages of B cell maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor β (TCRβ) during early T cell development. IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.