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商品編號


AG-40B-0093-C010



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

10 ?g


商品信息


More Information



Product Details



Synonyms

Leukemia Inhibitory Factor; Differentiation-stimulating Factor; D Factor; Melanoma-derived LPL Inhibitor



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human LIF (aa 23-202) is fused at the N-terminus to a tag.



Crossreactivity

Human
Mouse



Specificity

Mouse and human embryonic stem cells.



BIOLOG
ical Activity

Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from
ADI
pogen
has the same activity as cleaved LIF.



MW

~46kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.05eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.05eu>



Concentration


AG-40B-0093-C010/AG-40B-0093-3010:
0.1mg/ml after reconstitution.
AG-40B-0093-C100:
1mg/ml after reconstitution.



Reconstitution

Reconstitute with 100μl sterile water.



Formulation

Lyophilized. Contains PBS.



Other Product Data

UniProt link P15018:
LIF (human)




Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R α that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.