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商品編號


016-160-084



品牌


Jackson



公司


Jackson ImmunoResearch Laboratories



公司分類(lèi)


Streptavidin and Conjugates



Conjugate

Cyanine Cy?3


Unit

1.0 mg


Product

Streptavidin


產(chǎn)地

美國

商品信息

Steptavidin, a bacterial protein isolated from
Streptomyces avidinii
, is similar to egg-white avidin in its
ABI
lity to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.
Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.
Physical State:
Freeze-dried solid
Storage:

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.
Expiration date:
one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
St
ABI
lizer:
15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative:
0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
Western Blot:- 0.5-2 ?g / ml
Histo-/Cyto-Chemistry:-0.5-2 ?g / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, perme
ABI
lity, etc. The actual dilution used must be determined empirically.
Cyanine Cy?3
Amax:
550
Emax:
570nm
Cy3 is brighter, more photostable, and gives less background than other orange-red fluorescing dye conjugates. Cy3 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. For fluorescence microscopy, Cy3 can be visualized with tr
ADI
tional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. We recommend Cy3 as a brighter alternative to TRITC. Cy3 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). Cy3 has been used with fluorescein for double labeling; however, the use of a narrow band-pass emission filter for fluorescein is recommended to minimize Cy3 fluorescence in the FITC filter set. Cy3 can also be paired with Alexa Fluor? 647 for multiple labeling when using a confocal microscope. However, a better choice for multiple labeling is Rhodamine Red-X because its fluorescence is midway between a green fluorescing dye (like Alexa Fluor? 488) and a far-red-fluorescing dye like Alexa Fluor? 647.