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商品編號


016-030-084



品牌


Jackson



公司


Jackson ImmunoResearch Laboratories



公司分類(lèi)


Streptavidin and Conjugates



Conjugate

Horseradish Peroxidase


Unit

1.0 mg


Product

Streptavidin


產(chǎn)地

美國

商品信息

Steptavidin, a bacterial protein isolated from
Streptomyces avidinii
, is similar to egg-white avidin in its
ABI
lity to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.
Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.
Physical State:
Freeze-dried solid
Storage:

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.
Expiration date:
one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
St
ABI
lizer:
15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative:
None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horser
ADI
sh peroxidase.)
Suggested Working Concentration or Dilution Range:
ELISA
:- 1-2 ?g / ml
Western Blot:- 0.01-0.1 ?g / ml
Histo-/Cyto-Chemistry:- 1-2 ?g / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, perme
ABI
lity, etc. The actual dilution used must be determined empirically.
Horser
ADI
sh Peroxidase
Horser
ADI
sh peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974.
22
, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and
ELISA
. Affinity-purified anti-horser
ADI
sh peroxidase and conjugates are available for detection of horser
ADI
sh peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horser
ADI
sh peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.