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商品編號


016-470-084



品牌


Jackson



公司


Jackson ImmunoResearch Laboratories



公司分類(lèi)


Streptavidin and Conjugates



Conjugate

DyLight? 405


Unit

1.0 mg


Product

Streptavidin


產(chǎn)地

美國

商品信息

Steptavidin, a bacterial protein isolated from
Streptomyces avidinii
, is similar to egg-white avidin in its
ABI
lity to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.
Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.
Physical State:
Freeze-dried solid
Storage:

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.
Expiration date:
one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
St
ABI
lizer:
15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative:
0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
Western Blot:- 0.5-2 ?g / ml
Histo-/Cyto-Chemistry:-0.5-2 ?g / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, perme
ABI
lity, etc. The actual dilution used must be determined empirically.
DyLight? 405
Amax:
400
Emax:
421nm
DyLight 405-conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use is limited to microscopes or flow cytometers equipped with a 405 nm laser and a 420 nm emission filter. Under these conditions, it is poss
IBL
e to perform effective 4-color imaging with good color separation, good photost
ABI
lity, and high sensitivity. The combination of DyLight 405, Alexa Fluor? 488, Rhodamine Red-X, and Alexa Fluor? 647 provides for maximum color separation. Another 4-color dye combination, which may be equally effective but has slightly less color separation, is DyLight 405, Alexa Fluor? 488, Cy3, and Alexa Fluor? 647. DyLight 405 conjugates are an excellent choice for blue-fluorescing secondary antibodies in multi-color labeling protocols.