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商品編號


016-600-084



品牌


Jackson



公司


Jackson ImmunoResearch Laboratories



公司分類(lèi)


Streptavidin and Conjugates



Conjugate

Alexa Fluor? 647


Unit

1.0 mg


Product

Streptavidin


產(chǎn)地

美國

商品信息

Steptavidin, a bacterial protein isolated from
Streptomyces avidinii
, is similar to egg-white avidin in its
ABI
lity to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.
Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.
Physical State:
Freeze-dried solid
Storage:

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.
Expiration date:
one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
St
ABI
lizer:
15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative:
0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
Western Blot:- 0.5-2 ?g / ml
Histo-/Cyto-Chemistry:-0.5-2 ?g / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, perme
ABI
lity, etc. The actual dilution used must be determined empirically.
Alexa Fluor? 647
Amax:
651
Emax:
667nm
Alexa Fluor? 647-conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. They are brighter than Cy5 and DyLight 650 in aqueous mounting media. Alexa Fluor? 647- and APC-conjugated secondary antibodies are the best choice for flow cytometry when secondary antibodies fluorescing at these wavelengths are desired. Alexa Fluor? 647 conjugates are the best choice of far red-emitting dyes for multiple-labeling detection with a confocal microscope.
A significant advantage of using Alexa Fluor? 647 over lower wavelength-emitting dyes is the low autofluorescence of
BIOLOG
ical specimens in this region of the spectrum. However, because of its peak emission at 667 nm, Alexa Fluor? 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Therefore, Alexa Fluor? 647 is not recommended for use with conventional epifluorescent microscopes. It is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector. Alexa Fluor? 647 conjugates are less expensive alternatives to allophycocyanin conjugates for flow cytometry.