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商品編號


RF0059



品牌


Agrenvec



公司


Agrenvec



公司分類(lèi)


Anti-Human Polyclonal Serum



Size

100-μl-lyophilized

商品信息

Description:
?Rabbit Polyclonal Antibody Serum to?FLt3
Application:
?WB,
ELISA
Reactivity:
?Human
Conjugate:
Unconjugated
ELISA
:
?Suggested starting dilution 1/1,000 anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
Description:?
Anti-Human FLt3? Serum is developed in rabbit using recombinant rH FLt3? produced in plants.

Immunogen
: Highly pure (>97%) recombinant human FLt3 in expressed in plants.

Applications:


ELISA
: Suggested starting dilution 1/2,000. This antibody, in conjunction with compat
IBL
e secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of < of 10 ng/well? of rhuman FLt3? (RF0041).

Western Blot:
?Suggested starting dilution 1/1,000 anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.



Fig 1. Western Blot analysis of rhuman FLt3 using Anti-Human FLt3 Serum (RF0059)

Human FLt3? protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1: 2,000 of Anti-Human FLt3 Serum.? Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.25 ug f non glycosylated rHuman Follistatin protein produced in a prokaryotic expression system. Lane 3: 0.25 ug of? rH Flt3-ligand produced in the endosperm tissue of barley grain.? Lane 4: 0.5 ug of glycosylated isoforms of rH FLt3 produced in an eukaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures. Optimal dilution conditions should be determined by the final user. Bulk sizes are available upon request. For R&D purposes only. Purchaser must determine the suit
ABI
lity of the product(s) for their particular use.