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商品編號


RF0045



品牌


Agrenvec



公司


Agrenvec



公司分類(lèi)


Anti-Human Polyclonal Serum



Size

100-μl-lyophilized

商品信息

Description:
?Rabbit Polyclonal Antibody Serum to?IL12p40
Application:
?WB,
ELISA
Reactivity:
Unconjugated
ELISA
:
Suggested starting dilution 1/1,000. This antibody, in conjunction with compat
IBL
e secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of < 10 ng/well of rhuman IL12p40 (RF0031).
Western Blot:
?Suggested starting dilution 1/10,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
Description:
?Anti-Human IL12p40 Serum is developed in rabbit using recombinant IL12p40 produced in plants.

Immunogen
:?Highly pure (>97%) recombinant human IL12p40? expressed in plants.

Applications:


ELISA
:?
Suggested starting dilution 1/1,000. This antibody, in conjunction with compat
IBL
e secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of < 10 ng/well of rhuman IL12p40 (RF0031).

Western Blot:
?Suggested starting dilution 1/10,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.




Fig 1. Western Blot analysis of rhuman IL12p40 using Anti-Human IL12p40 Serum (RF0045)

Human IL12p40 protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:10,000 of Anti-Human IL12p40 Serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1 : MWM; Lane 2: 0.25 ug of glycosylated isoforms of rHuman IL-12p40 produced in an eukaryotic expression system. Lane 3: 0.25 ug? of non glycosylated rHuman IL-12p40 protein produced in a prokaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures. Optimal dilution conditions should be determined by the final user. Bulk sizes are available upon request. For R&D purposes only. Purchaser must determine the suit
ABI
lity of the product(s) for their particular use.