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商品編號


RF0096



品牌


Agrenvec



公司


Agrenvec



公司分類(lèi)


Anti-Human Polyclonal Serum



Size

100-μl-lyophilized

商品信息

Description:
?Rabbit Polyclonal Antibody Serum to sRANKL
Application:
?WB,
ELISA
Reactivity:
?Human
Conjugate:
?Unconjugated
ELISA
:
Suggested starting dilution 1/4,000. This antibody, in conjunction with compat
IBL
e secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of > 0,5 ng/well of rhuman sRANKL (RF0038)
Western Blot:
Suggested starting dilution 1/4,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
Description:
?Anti-Human sRANKL Serum is developed in rabbit using recombinant sRANKL produced in plants.

Immunogen
:?Highly pure (>97%) recombinant human?sRANKL expressed in plants.

Applications:


ELISA
:
Suggested starting dilution 1/4,000. This antibody, in conjunction with compat
IBL
e secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of > 0,5 ng/well of rhuman sRANKL (RF0038)

Western Blot:
Suggested starting dilution 1/4,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.


?

Fig 1. Western Blot analysis of rhuman sRANKL using Anti-Human sRANKL Serum (RF0096)

Human sRANKL? protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:4,000 of Anti-Human sRANKL serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM (kDa); Lane 2: 0.5 ug of glycosylated isoforms of rHuman sRANKL protein produced in an eukaryotic expression system. Lane 3: 0.1 ug of non glycosylated? rHuman sRANKL protein produced in a prokaryotic expression system.

Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user. Bulk sizes are available upon request. For R&D purposes only. Purchaser must determine the suit
ABI
lity of the product(s) for their particular use.