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商品編號


AG-40T-0106-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Probable Inorganic Polyphosphate/ATP-NAD Kinase; Poly(P)/ATP NAD Kinase; ppnK; yjbN; EC 2.7.1.23



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Full-length NAD kinase (
Bacillus subtilis
).



BIOLOG
ical Activity

~2.6 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1?mol nicotinamide adenine dinucleotide phosphate (NADP) per min.



MW

~30.0kDa



Purity

≥98% (SDS-PAGE)



Endotoxin Content

N/A



Concentration

Lot specific



Formulation

Liquid. In 50mM TRIS-HCl, pH 7.0 containing 150mM sodium chloride, 1mM EDTA and 1mM dithiothreitol.



Other Product Data

UniProt link O31612: NAD Kinase (B. subtilis)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD
+
to generate NADP
+
, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP
+
is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from
Bacillus subtilis
is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP
+
by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus.
Catalytic activity: ATP + NAD
+
= ADP + NADP
+



Product References

Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid: S. Garavaglia, et al.; J. Bacteriol.
185,
4844 (2003)