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商品編號


AG-40T-0090-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

NADK; Poly(P)/ATP NAD Kinase; EC 2.7.1.23



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human NAD kinase (aa 64-446) is fused at the N-terminus to a His-tag.



Crossreactivity

Human



BIOLOG
ical Activity

≥2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1?mol of NADP per min.



MW

~42kDa (monomer)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

N/A. Free of any nucleotide converting side-activities.



Concentration

1mg/ml



Reconstitution

Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH7.5, 150mM sodium chloride and 1mM DTT.



Formulation

Liquid. Ammonium sulphate s
USP
ension.



Other Product Data

UniProt link O95544: NAD kinase (human)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis.


Product References

Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC
288
, 69 (2001)

NADPH regulates human NAD kinase, a NADP+-biosynthetic enzyme: K. Ohashi, et al.; Mol. Cell Biochem.
355,
57 (2011)