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商品編號


AG-40T-0109-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

NH(3)-dependent NAD(+) Synthetase; General Stress Protein 38; GSP38; Spore Outgrowth Factor B; EC6.3.1.5



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Full-length NAD synthetase (
Bacillus subtilis
).



BIOLOG
ical Activity

~0.3 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1?mol NAD per min.



MW

~30.4kDa



Purity

≥98% (SDS-PAGE)



Endotoxin Content

N/A



Concentration

0.5mg/ml (Lot specific)



Formulation

Liquid. In 50mM TRIS-HCl, pH 8, containing 75mM sodium chloride, 5% glycerol and 5mM DTT.



Other Product Data

UniProt link P08164: NAD Synthetase (B. subtilis)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD
+
, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in
BIOLOG
ical processes like stress response and sporulation resulting in formation of a cellular spore.
Catalytic activity: ATP + deamido-NAD
+
+ NH3 = AMP + diphosphate + NAD
+


Product References

Crystal structure of NH3-dependent NAD+ synthetase from Bacillus subtilis: M. Rizzi, et al.; EMBO J.
15,
5125 (1996)