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商品編號


AG-40A-0017-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.



Crossreactivity

Mouse



BIOLOG
ical Activity

Shows
ADI
pogen
ic effects in stimulated differentiating 3T3-L1 cells.



MW

~52kDa (SDS-PAGE)



Purity

≥90% (SDS-PAGE)



Endotoxin Content

<1eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">1eu>



Reconstitution

Reconstitute in distilled water.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS, pH 7.2.



Other Product Data

UniProt link Q99KQ4: Nicotinamide phosphoribosyltransferase (mouse)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
For maximum product recovery after thawing, centrifuge the vial before opening the cap.



Use/St
ABI
lity

Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an
ADI
pokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.


Product References

Visfatin regulates insulin secretion, insulin receptor signalling and mRNA expression of diabetes-related genes in mouse pancreatic beta-cells: J.E. Brown, et al.; J. Mol. Endocrinol.
44,
171 (2010)