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商品編號


AG-40T-0021-C002



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

2 ?g


商品信息


More Information



Product Details



Synonyms

Nuclear Factor NF-κB p50 Subunit



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human NF-κB (p50) is fused to a His-tag.



Crossreactivity

Human



BIOLOG
ical Activity

~1ng is required for high mobility shift assay.
~5ng are required for reconstituted transcription assays.



Purity

≥95% (SDS-PAGE)



Concentration

Lot dependent (approx. 0.2mg/ml)



Formulation

Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.



Other Product Data

UniProt ID P19838:?NF-κB (human) (p50 subunit)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





NF-κB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many
BIOLOG
ical processes such as inflammation, immunity, differentiation, cell growth, tum
Origen
esis and apoptosis. NF-κB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at κB sites in the DNA of their target genes and the individual dimers have distinct preferences for different κB sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κB inhibitor (I-κB) family. In a conventional activation pathway, I-κB is phosphorylated by I-κB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κB complex which translocates to the nucleus. NF-κB heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-κB p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3.


Product References

Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem.
278,
45145 (2003)

Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem.
280,
40450 (2005)

Oxidized guanine base lesions function in 8-oxoguanine DNA glycosylase-1-mediated epigenetic regulation of Nuclear Factor κB-driven gene expression: L. Pan, et al.; J. Biol. Chem.
291,
25553 (2016)

OGG1-DNA interactions facilitate NF-κB binding to DNA targets: L. Pan, et al.; Sci. Rep.
7,
43297 (2017)