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商品編號


AG-40T-0092-3050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

3 x 50 ?g


商品信息


More Information



Product Details



Synonyms

Nicotinamide Mononucleotide Adenylyltransferase 1; NMN Adenylyltransferase 1; NaMN Adenylyltransferase 1; EC 2.7.7.1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human full-length NMNAT1 (aa 1-279) is fused at the N-terminus to a His-tag.



Crossreactivity

Human



Application

Well suited for the synthesis of NAD due to high specific activity and high substrate selectivity compared to NMNAT3 (human) (rec.) (His) (highly active). For NAD analog synthesis use NMNAT3 (human) (rec.) (His) (highly active) (AG-40T-0093).

Note:
When loss of activity is observed add 10mM DTT to the working buffer and incubate for at least 15 min before the assay.



BIOLOG
ical Activity

≥5U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1?mol of NAD
+
per min.



MW

~33.3kDa (monomer)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

N/A



Concentration

~1mg/ml



Formulation

Liquid. In 50mM sodium phosphate, pH8.0, containing 300mM sodium chloride, 2mM DTT and 10% glycerol.



Other Product Data

UniProt link Q9HAN9: NMNAT1 (human)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Nuclear NMNAT isoform. Catalyzes the formation of NAD
+
from nicotinamide mononucleotide (NMN) and ATP. It can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Interacts with PARP-1/ARTD1. Protects against axonal degeneration following mechanical or toxic insults. Widely expressed.


Product References

Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase: M. Emanuelli, et al.; J. Biol. Chem.
276,
406 (2001)

Characterization of recombinant human nicotinamide mononucleotide adenylyl transferase (NMNAT), a nuclear enzyme essential for NAD synthesis: M. Schweiger, et al.; FEBS Lett.
492,
95 (2001)

Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem.
280,
36334 (2005)

The NMN/NaMN adenylyltransferase (NMNAT) protein family: C. Lau, et al.; Front. Biosci.
14,
410 (2009)

Isoform-specific targeting and interaction domains in human nicotinamide mononucleotide adenylyltransferases: C. Lau, et al.; J. Biol. Chem.
285,
18868 (2010)