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商品編號


JAI-OPNUCP1



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

500 Units

商品信息


More Information



Product Details



Product Type

Protein



Properties



Sequence

A zinc dependent glycoprotein consisting of 270 amino acid residues.



Application

Used for DNA samples pretreatment to detect 8-OHdG.



BIOLOG
ical Activity

3′-5′-Phosphodiesterase: One unit will liberate ~1.0μmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3′-Nucleotidase: One unit will hydrolyze ~4.0μmol of orthophosphate from 3′-AMP per min at pH 7.2 at 37°C.



MW

42-50 kDa



Formulation

Lyophilized.



Other Product Data

The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.

Optimal pH:
5.3 (RNA and heat-denatured DNA); 7.2 (3'-AMP)

Heat St
ABI
lity:
Stable below 60°C at pH 5.3 for 30 minutes.



Declaration

Manufactured by JaICA.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at +4°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phospho-oligonucleotides. Nuclease P1 hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat-denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mmol/l of sodium chloride at pH 6.0.