Adipogen/PARP-1 [ARTD1] (catalytic domain) (human) (rec.) (His)/AG-40T-0010-C020/20 ?g
重組蛋白
商品編號
AG-40T-0010-C020
品牌
Adipogen
公司
Adipogen
公司分類(lèi)
Proteins
Size
20 ?g
商品信息
More Information
Product Details
Synonyms
Poly(ADP-ribose) Polymerase 1; ADP-ribosyltransferase Diphtheria Toxin-like 1; NAD(+) ADP-ribosyltransferase 1
Product Type
Protein
Properties
Source/Host
Sf21 cells
Sequence
Human PARP-1 [ARTD1] (aa 656-1014) is fused to a MYC and His-tag.
Crossreactivity
Human
BIOLOG
ical Activity
~50ng are required for poly(ADP-ribose) formation.
Purity
≥90% (SDS-PAGE)
Concentration
Lot dependent (0.2-1.5mg/ml)
Formulation
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM sodium chloride, 250mM imidazole, 2mM β-mercaptoethanol, 0.2% NP-40 and 10% glycerol.
Other Product Data
UniProt ID P09874:?PARP-1 (human)
Shipping and Handling
Shipping
DRY ICE
Short Term Storage
-20°C
Long Term Storage
-80°C
Handling Advice
After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.
Use/St
ABI
lity
Stable for at least 6 months after receipt when stored at -80°C.
Documents
MSD
S
Download PDF
Product Specification Sheet
Datasheet
Download PDF
PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway le
ADI
ng to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-γ to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
Product References
The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function: P.O. Hassa, et al.; J. Biol. Chem.
276,
45588 (2001)
產(chǎn)品貨號:4268.0