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商品編號


AG-40T-0556-C100



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

PTEN-induced Putative Kinase 1; Serine/Threonine-protein Kinase PINK1, Mitochondrial; PARK6; Parkinson Disease (Autosomal Recessive) 6; BRPK; EC 2.7.11.1; FLJ27236



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

PINK1 (Tribolium castaneum) (Accession Nr. XP_968367.1) fused to a N-terminal MBP and His-tag.



Crossreactivity

Human



MW

~108kDa



Purity

≥90% (SDS-PAGE)



Formulation

Liquid. In 20mM HEPES pH7.6, 50mM NaCl, 10% (v/v) Glycerol, 1mM TCEP.



Other Product Data


Use:
We recommend an initial PINK1 concentration of 0.5-2μM for the phosphorylation of recombinant Parkin, Ubiquitin or Polyubiquitin chains. Reaction conditions will need to be optimized for each specific application.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress. PINK1 is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria PINK1 becomes st
ABI
lized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Mfn2. Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by PINK-mediated phosphorylation of PARK2 at serine 65, and PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65). This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2.
Recombinant human PINK1 is not active in vitro, while this protein produced from the Red Flour Beetle (Tribolium castaneum) effectively phosphorylates recombinant Parkin, mono-Ubiquitin, and poly-Ubiquitin chains. It specifically phosphorylates both Parkin and Ubiquitin at serine 65.