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Adipogen/Poly-SUMO3 Hydrolysis Resistant (3-8) (human) (rec.)/AG-40T-0323-C050/50 ?g

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Adipogen/Poly-SUMO3 Hydrolysis Resistant (3-8) (human) (rec.)/AG-40T-0323-C050/50 ?g


商品編號


AG-40T-0323-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Small Ubiquitin-related Modifier 3; HSMT3; SMT3 Homolog 1; Ubiquitin-like Protein SMT3B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

3-8 human SUMO3 Q89P mutants (Accession Nr. P55854).



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl and DTT.



Other Product Data


Use:
Formed enzymatically with recombinant SUMO3 containing a mutation of glutamine 89 to proline. Mono- and di-SUMO3 have been removed from the chain mixture. 500-fold more resistant to SENP disassembly than wild type chains and may be useful for investigating SUMO-binding proteins. Reaction conditions will need to be optimized for each specific application.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Small Ubiquitin-like Modifier 3 (SUMO3), also known as SMT3A, is synthesized as a 103 amino acid (aa), propeptide with a predicted 11.5 kDa. SUMO3 contains a two aa C-terminal prosegment. Human SUMO3 shares 83% sequence identity with mouse SUMO3. Di-SUMO3 represents two wild-type recombinant human SUMO3 molecules linked via Lys11, which is the point of attachment for the C-terminal glycine residue of the preceding SUMO3. Di-SUMO3 can be used as a substrate for SUMO-specific isopeptidases (SENPs) and DeSUMOylating Isopeptidase 1 that cleave the isopeptide linkage between two adjacent SUMO3 molecules. Di-SUMO3 can also be used to investigate mechanisms of binding and recognition by SUMO-activating (E1) enzymes, SUMO-conjugating (E2) enzymes, SUMO E3 ligases and other proteins that contain SUMO binding domains. SUMOs are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation. Unlike SUMO1 which is usually conjugated to proteins as a monomer, SUMO2 and SUMO3 form high molecular weight polymers on proteins. All SUMO proteins share a conserved ubiquitin domain and a C-terminal diglycine cleavage/attachment site. Following prosegment cleavage, the C-terminal glycine residue of SUMO3 is enzymatically attached to a lysine residue on a target protein.

上一篇 GenWay/ERBB-3 Receptor Protein Tyrosine Kinase (Her3) (ERBB3) Goat anti-Human Polyclonal (C-Terminus) Antibody/GWB-5A725  下一篇 GenWay/Epidermal Growth Factor Receptor (EGFR) Rabbit anti-Human Polyclonal (pTyr1197) Antibody/GWB-4C81D8/0.05 mg

產(chǎn)品貨號:3450.4

3450.4 ¥
11至15個(gè)工作日送達
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