Adipogen/Poly-SUMO3 Hydrolysis Resistant (3-8) (human) (rec.)/AG-40T-0323-C050/50 ?g
重組蛋白
商品編號
AG-40T-0323-C050
品牌
Adipogen
公司
Adipogen
公司分類(lèi)
Proteins
Size
50 ?g
商品信息
More Information
Product Details
Synonyms
Small Ubiquitin-related Modifier 3; HSMT3; SMT3 Homolog 1; Ubiquitin-like Protein SMT3B
Product Type
Protein
Properties
Source/Host
E. coli
Sequence
3-8 human SUMO3 Q89P mutants (Accession Nr. P55854).
Crossreactivity
Human
Formulation
Liquid. In HEPES, NaCl and DTT.
Other Product Data
Use:
Formed enzymatically with recombinant SUMO3 containing a mutation of glutamine 89 to proline. Mono- and di-SUMO3 have been removed from the chain mixture. 500-fold more resistant to SENP disassembly than wild type chains and may be useful for investigating SUMO-binding proteins. Reaction conditions will need to be optimized for each specific application.
Declaration
Manufactured by Boston Biochem
Shipping and Handling
Shipping
DRY ICE
Short Term Storage
-20°C
Long Term Storage
-80°C
Handling Advice
Aliquot to avoid freeze/thaw cycles.
Use/St
ABI
lity
Stable for at least 1 year after receipt when stored at -80°C.
Documents
MSD
S
No
Product Specification Sheet
Datasheet
Download PDF
Small Ubiquitin-like Modifier 3 (SUMO3), also known as SMT3A, is synthesized as a 103 amino acid (aa), propeptide with a predicted 11.5 kDa. SUMO3 contains a two aa C-terminal prosegment. Human SUMO3 shares 83% sequence identity with mouse SUMO3. Di-SUMO3 represents two wild-type recombinant human SUMO3 molecules linked via Lys11, which is the point of attachment for the C-terminal glycine residue of the preceding SUMO3. Di-SUMO3 can be used as a substrate for SUMO-specific isopeptidases (SENPs) and DeSUMOylating Isopeptidase 1 that cleave the isopeptide linkage between two adjacent SUMO3 molecules. Di-SUMO3 can also be used to investigate mechanisms of binding and recognition by SUMO-activating (E1) enzymes, SUMO-conjugating (E2) enzymes, SUMO E3 ligases and other proteins that contain SUMO binding domains. SUMOs are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation. Unlike SUMO1 which is usually conjugated to proteins as a monomer, SUMO2 and SUMO3 form high molecular weight polymers on proteins. All SUMO proteins share a conserved ubiquitin domain and a C-terminal diglycine cleavage/attachment site. Following prosegment cleavage, the C-terminal glycine residue of SUMO3 is enzymatically attached to a lysine residue on a target protein.
產(chǎn)品貨號:3450.4