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商品編號


AG-40T-0328B-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

2-7 human ubiquitin (Accession Nr. P0CG47) covalently linked through isopeptide bonds at K48 residues of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule and conjugated to biotin.



Crossreactivity

Human



Label/Conjugates

Biotin



Formulation

Lyophilized from a solution in HEPES.



Other Product Data


Use:
This mixture of poly-ubiquitin chains contains di-ubiquitin and higher MW species, mono-ubiquitin has been removed. Modified with biotin via primary amine coupling which results in multiple biotinylated species modified at the N-terminus, as well as lysine residues. Biotinylated ubiquitin can be detected using avidin-linked reagents. Ideal for use in assays that utilize avidin-linked reagents for visualization or quantitation. Reaction conditions will need to be optimized for each specific application.
IMPORTANT:
Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40°c for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">40°c>



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Poly-ubiquitin chains are composed of ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule. Each human ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse ubiquitin, respectively. Seven of the 76 aa in ubiquitin are lysine residues that can participate in poly-ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking and other cellular processes. Linkage-specific poly-ubiquitin chains are used to investigate mechanisms of chain recognition, binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Lys48-linked chains are abundant
in vivo
and act as a universal signal for proteasomal degradation.