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商品編號


GWB-8548C0



品牌


GenWay



公司


GenWay



公司分類(lèi)


Secondary Antibodies



Size

0.5 mg

商品信息

Additional Information:




Name

Goat F(ab')2 Anti-Human IgG ( chain specific) Absorbed against mouse immunoglobulins



Related Product Names

Goat F(ab')2 Anti-Human IgG, Mouse ads-BIOT; N/A Goat F(ab')2 Anti-Human IgG ( chain specific) Absorbed against mouse immunoglobulinsIGHG1



Gene Symbol

IGHG1



Gene Fullname

Ig gamma-1 chain C region



Protein Name

Ig gamma-1 chain C region



Swissprot ID

P01857



Host

R- phycoerythrin (R- PE) Conjugate



Isotype

IgG



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



Product Format

Biotin (BIOT) Conjugate



Swiss Prot Number

P01857



Purification

Gel filtration chromatography of pepsin digested antibody.



Specificity

Reacts with the heavy chain of human IgG as demonstrated by
ELISA
, FLISA,and/or flow cytometry. Minimal cross reactivity with mouse immunoglobulins.May react with IgG from other species.



Applications

IF of IgG+,
ELISA
, WB, DB, IHC



Application Info

- Direct immunofluorescent staining of IgG
+
human B lymphocytes
- Enzyme-Linked-Immunosorbent-Assay (
ELISA
)
- Fluorescent-Linked-Immunosorbent-Assay (FLISA)
- Western blotting
- Dot-and slot-immunoblotting
- Immunohistochemi



Dilution

Immunofluorescence:
ELISA
:
Purified antibody
FITC
BIOT conjugate
R-PE conjugate
BIOT conjugate
<= 1="">=>
6
cells
<= 1="">=>
6
cells
<= 1="">=>
6
cells
<= 0.1="">=>
6
cells
1:5,000-1:20,000



Clonality

Polyclonal



Format

Biotin (BIOT) Conjugate



Absorption

Human IgM and IgA, pooled mouse sera and mouse immunoglobulins.



Reactivity

Human



Warning

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.



Storage

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8C.
The fluorescein (FITC) conjugate is supplied as 0.5 m



Protocol

Reconstitution Instructions
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme- Linked ImmunoSorbent Assay (
ELISA
) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only