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商品編號


AG-40T-0554-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin C-terminal Hydrolase 30; Deubiquitinating Enzyme 30; Ubiquitin Thioesterase 30; Ubiquitin-specific-processing Protease 30; EC 3.1.2.15; EC 3.4.19.12



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human
USP
30 (aa57-517) (Accession Nr. Q70CQ3) fused to a C-terminal His-tag.



Crossreactivity

Human



MW

~54kDa



Purity

≥98% (SDS-PAGE)



Formulation

Liquid. In 50mM HEPES pH7.2, 400mM NaCl, 10% Glycerol (v/v), 2mM TCEP.



Other Product Data


Use:
Reaction conditions will need to be optimized for each specific application. We recommend an initial
USP
30 concentration of 20-100nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates, or 200-500nM when digesting recombinant polyubiquitin chain substrates.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin carboxyl-terminal hydrolase 30 (
USP
30) is a member of the C19 family of peptidases and is tethered to the outer mitochondrial surface via a trans-membrane domain.
USP
30 activity is antago
NIST
ic to the pro-mitophagy signal generated by the activities of E3 Ubiquitin Ligase Parkin (PARK2) and kinase PINK1, which are stimulated by mitochondrial damage.
In vivo
,
USP
30 cleaves Ubiquitin from mitochondrial surface proteins such as MIRO1, TOMM20, MFN1 and MFN2. The enzyme displays a strong preference for K6- and K11-linked polyubiquitin chains
in vitro
, but has substantially lower activity on polyubiquitin chains that have been phosphorylated with PINK1 kinase.