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商品編號


GWB-85FD00



品牌


GenWay



公司


GenWay



公司分類(lèi)


Secondary Antibodies



Size

0.5 mg

商品信息

Additional Information:




Name

Rabbit F(ab')2 Anti-Mouse IgG (H+L) Absorbed against human immunoglobulins



Related Product Names

Rabbit F(ab')2 Anti-Mouse IgG(H+L), Human ads-UNLB; N/A Rabbit F(ab')2 Anti-Mouse IgG (H+L) Absorbed against human immunoglobulinsIGHG1



Gene Symbol

IGHG1



Gene Fullname

Ig gamma-1 chain C region



Protein Name

Ig gamma-1 chain C region



Swissprot ID

P01857



Host

Pepsin digest of rabbit anti- mouse IgG (H+L).



Isotype

IgG



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



Product Format

Purified (UNLB) Antibody



Swiss Prot Number

P01857



Purification

Affinity chromatography on mouse IgG covalently coupled to agarose. Gelfiltration chromatography of pepsin digested antibody.



Specificity

Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, andwith the light chains of mouse IgM and IgA as demonstrated by
ELISA
and flowcytometry. Minimal cross reactivity with human immunoglobulins.



Applications

IF,
ELISA
, WB, DB, IHC



Application Info

- Indirect immunofluorescent staining in conjunction with mouse monoclonal antibodies
- Enzyme-Linked-Immunosorbent-Assay (
ELISA
)
- Western blotting
- Dot-and slot-immunoblotting
- Immunohistochemistry



Dilution

Immunofluorescence:
ELISA
:
FITC conjugate
AP conjugate
HRP conjugate
<= 1="">=>
6
cells
1:2,000-1:4,000
1:4,000-1:8,000



Clonality

Polyclonal



Format

Purified (UNLB) Antibody



Absorption

Pooled human sera and purified human paraproteins.



Reactivity

Mouse



Warning

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.



Storage

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8C.
The fluorescein (FITC) conjugate is supplied as 0.5 m



Protocol

Reconstitution Instructions
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme- Linked ImmunoSorbent Assay (
ELISA
) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only