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商品編號


AG-40T-0043-C020



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

20 ?g


商品信息


More Information



Product Details



Synonyms

Vascular Endothelial Growth Factor; VEGF-A; VPF



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human VEGF 165 (165aa).



Crossreactivity

Human



BIOLOG
ical Activity

The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF 165 has been determined to be in the range of 1-5ng/ml.



MW

~38.2kDa



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.1ng mu;g="" protein.="">0.1ng>



Reconstitution

Reconstitute with 50mM acetic acid to a concentration not lower than 50 μg/ml.



Formulation

Lyophilized.



Other Product Data

UniProt link P15692-4: VEGF 165 (human)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
For long term storage we recommend to add at least 0.1% human or bovine serum albumin.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Human vascular endothelial growth factor (VEGF 165) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. VEGF 165 is a specific mitogen for vascular endothelial cells and a strong angiogenic factor
in vivo
. In addition to its action as a mitogen it is a potent vascular perme
ABI
lity factor (VPF)
in vivo
. It is also a chemoattractant molecule for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces perme
ABI
lization of blood vessels.


Product References
Developmental coronary maturation is disturbed by aberrant cardiac vascular endothelial growth factor expression and Notch signalling: N.M.S. van den Akker, et al.; Cardiovasc. Res.
78
, 366 (2008)