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商品編號


16848



品牌


AAT Bioquest



公司


AAT Bioquest



公司分類(lèi)


Fluorescent Anti-IgGs



Size

1 mg


商品信息



Overview



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Ex/Em (nm)
330/544



MW
N/A



CAS #
N/A



Solvent
Water



Storage

F/D/L



Category
Immunology
Fluorescent Anti-IgGs




Related
iFluor? Dyes and Kits
Tag Antibodies
Biochemical Assays




Many
BIOLOG
ical compounds present in cells, serum or other
BIOLOG
ical fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the
BIOLOG
ical molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences. Our trFluor? Tb probes enable time-resolved fluorometry (TRF) for the assays that require high sensitivity. trFluor? Tb probes have large Stokes shifts and extremely long emission half-lives when compared to more tr
ADI
tional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our trFluor? Tb probes have relatively high st
ABI
lity, high emission yield and
ABI
lity to be linked to biomolecules. This trFluor? Tb goat anti-rabbit IgG (H+L) conjugate is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with primary antibodies.



Spectrum

Advanced Spectrum Viewer




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Move mouse over grid to display wavelength & intensity values.



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References & Citations




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1.???Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J. (2012) Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays. Assay Drug Dev Technol.

2.???Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH. (2012) Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors. Anal Biochem, 421, 368.

3.???Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A. (2011) Oligomerization of the
SERO
tonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach. J Phys Chem B, 115, 11439.

4.???Martikkala E, Rozwandowicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H. (2011) A homogeneous single-label time-resolved fluorescence cAMP assay. J Biomol Screen, 16, 356.

5.???Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A. (2011) Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies. J Biol Chem, 286, 11337.

6.???Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M'Kadmi C, Martinez J, Mary S, Baneres JL, Marie J. (2011) Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a. Anal Biochem, 408, 253.

7.???Posokhov YO, Kyrychenko A, Ladokhin AS. (2010) Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation. Anal Biochem, 407, 284.

8.???Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G. (2010) Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET. J Biol Chem, 285, 23318.

9.???Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD. (2010) A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization. Assay Drug Dev Technol, 8, 96.

10.???Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW. (2010) Time-resolved FRET fluorescence spectroscopy of vis
IBL
e fluorescent protein pairs. Eur Biophys J, 39, 241.



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