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商品編號


HTR1-100



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Protease



Concentration

1mg/ml


Size

1 mg

商品信息
Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen. It contains a signal peptide (residues 1?15), a pro region (residues 16?23), and a mature chain (residues 24?247).


Introduction
Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen. It contains a signal peptide (residues 1?15), a pro region (residues 16?23), and a mature chain (residues 24?247). Trypsin is part of the serine protease family. Trypsin cleaves lysine and arginine at the C-terminal side of the peptide. Trypsin optimum pH is pH-7 to 9. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g. EDTA.


Product Description
Recombinant Human Trypsin-1 is a genetically engineered protein expressed in
E. coli
and purified by standard chromatography techniques. Recombinant Human Trypsin-1 is free from any animal and human sources. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.


Source:
Human trypsin-1, expressed in
E. coli
, with a C-terminal 6-His tag.

Purity:
>95% as determined by SDS-PAGE


Lane:
1.
MCLAB
's Trypsin
2. Trypsin from supplier S



Unit Definition:
One
USP
unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


Specific activity:
≥ 2500
USP
U/mg


Storage:
Store at -20°C. ?Avoid multiple freeze-thaw cycles.