MCLAB/SUMO Protease/SP-100/25,000 U
酶
商品編號
SP-100
品牌
MCLab
公司
MCLAB.Inc
公司分類(lèi)
Protease
Concentration
100 U/μl
Size
25,000 U
商品信息
SUMO Protease has greater than 95% purity with no non-specific protease contamination. It recognizes the tertiary structure of SUMO and cleaves SUMO from recombinant fusion proteins.
Description:
SUMO (
S
mall
U
biquitin-like
MO
difiers) Protease is a highly purified Ulp1 from a
E. coli
expression system, which carries the yeast Ulp1. It recognizes the tertiary structure of SUMO and cleaves SUMO from recombinant fusion proteins. The SUMO protease has a N-terminal His-tag and can be removed by Ni-NTA agarose.
Application:
- Removal of fusion tags from recombinant proteins
- Highly dynamic and precise cleavage cap
ABI
lities
- Purification of proteins and peptides
Supplied with:
10X high salt and 10X no salt SUMO buffers
10× SUMO Protease Buffer (Salt plus)
500 mM Tris-HCl,pH 8.0
2% Igepal(NP-40)
1.5 M NaCl
10 mM DTT
10× SUMO Protease Buffer (Salt free)
500 mM Tris-HCl,pH 8.0
2% Igepal(NP-40)
10 mM DTT
SUMO Protease Storage Buffer
25 mM Tris-HCl,pH 8.0
0.1% Igepal(NP-40)
250 mM NaCl
0.5 mM DTT
50% glycerol?
Source:
E. coli
M.W.:
26 kDa
Purity:
≥95%
Label:
His-tag
Activity:
>10
5
Unit/mg
Quality Control:
SUMO Protease has greater than 95% purity with no non-specific protease contamination. It is functionally tested for the absence of any non-specific protease activity.
Unit Definition:
One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2?g of substrate protein at 30°C in one hour.
Recommended Storage Condition:
-80°C
Figures:
Figure 1, Coomassie staining of SUMO Protease in different elution fractions.
Figure 2, RP-HPLC Chromatogram of SUMO Protease.
產(chǎn)品貨號:2756.0