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商品編號


TEP-100



品牌


MCLab



公司


MCLAB.Inc



公司分類(lèi)


Protease



Concentration

1mg/ml


Size

1 mg


商品信息

TEV (Tobacco etch virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful reagent for cleaving fusion proteins due to its high specificity and high activity rate.





Description:
TEV (Tobacco Etch Virus) Protease is a highly site-specific cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. TEV protease is a very useful enzyme for cleaving fusion proteins due to its high specificity and its high activity rate. TEV Protease contains an N-terminal His-tag, and it can be removed by Ni-NTA column after cleavage reaction.
Application:
- Removal of fusion tags from recombinant proteins
- Highly dynamic and precise cleavage cap
ABI
lities
- purification of proteins and peptides
Supplied With:
TEV substrate, 50 ?l
10X TEV buffer
Source:
E. coli
derived from Tobacco Etch Virus.
Label:
His-tag
Reaction Conditions:
TEV protease is maximally active at 34°C, but its recommended to perform digests at room temperature (20°C) or 4°C. The activity of TEV protease is approximately 3-fold greater at 20°C than at 4°C.
10X TEV buffer
500 mM Tris-HCl, 1.5 M NaCl, pH 8.0
Recommended Storage Condition:
-20°C
Figures:
Figure 1
Figure 2, 69.8 kDa TEV substrate protein is incubated with TurboTEV. The cleaved products are 41.2 kDa and 27.7 kDa, respectively.